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Image Search Results
Journal: Kidney international
Article Title: Measurement of cytosolic and mitochondrial pH in living cells during reversible metabolic inhibition.
doi: 10.1038/sj.ki.5002632
Figure Lengend Snippet: Figure 6 | In situ calibration of SNARF-1 in MDCK cells. Cells were loaded (loading Protocol 2) with SNARF-1 and MTG and then incubated in high K þ-buffer supplemented with nigericin (13 mM), FCCP (1 mM), and oligomycin (20 mg/ml). Averaged SNARF-1 ratios (680/590 nm)±s.e.m. for cytosol (J; N ¼ 5, n ¼ 48) and mitochondria (’; N ¼ 5, n ¼ 48) were plotted against the pH of the bathing solution.
Article Snippet: In situ calibration: In situ calibration of SNARF-1 was carried out in separate experiments using the method of Thomas et al.39 MDCK cells were loaded according to Protocol 2 with SNARF-1 in the cytosol and with SNARF-1 plus MTG in mitochondria and exposed to high-Kþ buffer (vide infra Solutions and Chemicals) containing also 13 mM nigericin, 1 mM FCCP, and 20 mg ml 1
Techniques: In Situ, Incubation